Reference transcriptome

The orchid family is one of the largest families of flowering plants with over 25,000 recognised species, but within Orchidaceae there are only limited available genomic resources. High-throughput RNA sequencing has emerged as a powerful and cost-effective way to characterize gene content and identify polymorphic molecular markers in non-model organisms. However, assembling a transcriptome without a known reference remains a challenging task due to the complexity of RNA populations. Using an in-house developed pipeline, we have assembled reference leaf transcriptomes for two European terrestrial orchids, diploids Dactylorhiza incarnata and D. fuchsii (in subtribe Orchidinae).